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To Do:

Miniprep stuff

  •  Miniprep PY, LY, pENTR_TEVpCofilin
  •  Verify Minipreps

...

  •  Transform hEF1a:YFPCofilin

...

  •  Pick TREt:YFPCofilin Colonies
  •  LR pENTR_TEVpCofilin

 

3/4 pENTR_TEVpCofilin worked!

Gel

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Of course, the others didn't work. Given the lack of blue colonies, I'm thinking backbone religation/only one part present (probably YFP). Need to figure out something else.

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