Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Expand
titleParts Needed

BCR (CD79A/B, IgM heavy, IgM light), Lyn-mKate

 

anti IgM

Expand
titleSetup

Well 1

HEK293

 

Well 2

HEK293

eBFP

Dummy DNA

Well 3

HEK293

eBFP

Lyn-mKate

Dummy DNA

Well 4

HEK293

eBFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Well 5 

EMPTY

 

Well 6 

EMPTY

 

Well 7

HEK293

Oligomerized Beta Amyloid

Well 8 

HEK293

eBFP

Dummy DNA

Oligomerized Beta Amyloid

Well 9 

HEK293

eBFP

Lyn-mKate

Dummy DNA

Oligomerized Beta Amyloid

Well 10 

HEK293

eBFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Oligomerized Beta Amyloid

Well 11 

EMPTY

 

Well 12 

EMPTY

 

Well 13

HEK293

Monomerized Beta Amyloid

Well 14

HEK293

eYFP

Dummy DNA

Monomerized Beta Amyloid

Well 15

HEK293

eYFP

Lyn-mKate

Dummy DNA

Monomerized Beta Amyloid

Well 16

HEK293

eYFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Monomerized Beta Amyloid

Well 17

EMPTY


Well 18

EMPTY


Well 19

HEK293

Anti IgM

Well 20

HEK293

eYFP

Dummy DNA

Anti IgM

Well 21

HEK293

eYFP

Lyn-mKate

Dummy DNA

Anti IgM

Well 22

HEK293

eYFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Anti IgM

Well 23

EMPTY


Well 24

EMPTY

Produce two samples of cells with all parts, anti IgM +/- and look at with a microscope


Expand
titleExpected Results

Lyn should be membrane localized.  We will be lucky if Lyn only membrane localizes in anti IgM+ sampleslocalize in at least some samples. Hopefully Lyn will only localize if the BCR is active and will not localize if it is inactive.

Expand
titleProtocol

 

Expand
titleData

 

...