Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- What's the deal with the GAL4VP16?!
miRNA Sensors (Lab Work)
- Pick coloniesMini prep
- pEXPR: MAV1212-TRE-L7ae (mini)
- Digest and run gel
- pEXPR: MAV1212-hEF1a-GAL4VP16 (midi)TRE-L7ae
- Inoculate midi (if digest is okay)
- pEXPR: MAV1212-TRE-GAL4VP16 (midi)
- L7ae
- Midi prep
- MAV1212-hEF1a-TAL21GAL4VP16
- MAV1212-hEF1a-TAL14
- TRE-GAL4VP16
- All the single input sensors
- Pick colonies
- MAV1212-hEF1a-TAL21 (LR)
- MAV1212-hEF1a-TAL14 (LR)
- Plasmids from BCR group
- Plasmids from protein receptor group
- Plasmids from Brian
- Miniprep
- All the sensors (round 2)
- All the sensors (round 2)
- Analyze sequencing results
- All the sensors (round 1)
- Low Sensor miR-144-181c
- Transfect
- L7ae TAL14 Experiment (Take 31)
- Seed
- TAL14 Experiment #3 (Take 1)
- miR-144-181c double input target site
- Golden Gate
- Low Sensor miR-144-181c
- Inoculate midi cultures
- Each of the single input sensors
NOTES
All of the single input sensors were verified!
1st full day with no hiccups?
- Experiment #4 (Take 2)
NOTESGave constructs and plasmids maps (Kturns, L7ae, and MAV RFP) to Jeremy