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Goal: Test the strength of different repressors

NameOnOffInducible
L7ae
  •  Gateway Hef1a-2x Kturn-eYFP with pENTR_L1_L7ae_L2
  •  Co transfect w/ constituative eBFP2
  •  Flow Cytometry and analysis
  •  Just Hef1a-2x Kturn-eYFP
  •  Co transfect w/ constituative eBFP2
  •  Flow Cytometry and analysis
  •  rtTA & DOX?
TAL14
  •  Gateway Hef1a-2x Kturn-eYFP with pENTR_L1_TAL14_L2
  •  Co transfect w/ constituative eBFP2
  •  Flow Cytometry and analysis
  •  Just Hef1a-2x Kturn-eYFP
  •  Co transfect w/ constituative eBFP2
  •  Flow Cytometry and analysis
  •  rtTA & DOX?

Notes:

2x-Kturn-eYFP from Nelson

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