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Results

 

Lane12345678910111213141516
tubeHyper Ladder 1kbGG DONRpENTRSyk-eYFP 61pENTRSyk-eYFP 61pENTRSyk-eYFP 72pENTRSyk-eYFP 83pENTRSyk-eYFP 94pENTRSyk-eYFP 105pENTR Lyn TEV 61pENTR Lyn TEV 61pENTR Lyn TEV 72pENTR Lyn TEV 83pENTR Lyn TEV 94pENTR Lyn TEV 105GG DONRHyper Ladder 1kb
enzyme NsiIundigested controlNsiINsiINsiINsiINsiIundigested controlPvuIIPvuIIPvuIIPvuIIPvuIIPvuII 
Result(P/F)                
Notes                
Lane212223242526272829303132333435
TubeHyper Ladder 1kbGG DONRpENTR Lyn-mKate 61pENTR Lyn-mKate 61pENTR Lyn-mKate 72pENTR Lyn-mKate 83pENTR Lyn-mKate 94pENTR Lyn-mKate 105pENTR Lyn-stop 61pENTR Lyn-stop 61pENTR Lyn-stop 72pENTR Lyn-stop 83pENTR Lyn-stop 94pENTR Lyn-stop 105Hyper Ladder 1kb
Enzyme PvuIIundigested controlPvuIIPvuIIPvuIIPvuIIPvuIIundigested controlPvuIIPvuIIPvuIIPvuIIPvuII 
Result(P/F)               

 

 

Virtual gels from Geneious

pENTR Syk-eYFP
pENTR Lyn-TEV
pENTR Lyn+STOP
pENTR Lyn-mKate
Fail state (ggDONR)

 

 

 

 

 

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