"""
Escherichia coli TOP10 (Invitrogen) was used in this study. The plasmids used are pLOI297 harbouring the Zymomonas mobilis genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) cloned in pUC18 collinear to the lacZ promoter (Alterthum & Ingram, 1989), and pKS : : atfA and pBBR1MCS-2 : : atfA harbouring the WS/DGAT gene from A. baylyi strain ADP1 collinear to the lacZ promoter in pBluescript KS− or pBBR1MCS-2, respectively (Kalscheuer & Steinbüchel, 2003). The construction of plasmid pMicrodiesel is described in Results.
""" (http://mic.sgmjournals.org/content/152/9/2529.full, 2006)
"""
Specifically, we overexpressed two E. coli genes to promote free fatty acid accumulation: ‘tesA, a cytosolic ACP-thioesterase that cleaves acyl chains from acyl-ACP, thereby deregulating fatty acid synthesis and releasing free fatty acids into the cytosol ( Lu et al., 2008 and Steen et al., 2010), and fadR, a transcriptional regulator that controls the expression of several E. coli fatty acid synthase genes ( Zhang et al., 2012).
""" (http://www.sciencedirect.com.ezproxyberklee.flo.org/science/article/pii/S1096717614001165, 2014)