How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Double input low sensor??
- Where are the siRNA?
miRNA Sensors (Lab Work)
- Mini prep
- miR-30d (TAL14)
- miR-16a (TAL14)
- let-7f (TAL14)
- pEXPR: hEF1a-TAL21
- Send for sequencing
- miR-30d (TAL14)
- miR-16a (TAL14)
- let-7f (TAL14)
- Digest and Run gel
- MAV1212-hEF1a-TAL21
- Flow Cytometry
- L7ae tuning experiment
- Transform
- miR-125b (TAL14)
NOTES
minipreps took a really long time --> TAL21 wasn't digested
siRNA should be coming "soon"
since HS_125b didn't grow colonies --> re transformed