How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Can we quantify repression?
miRNA Sensors (Lab Work)
- Analyze sequencing results
- miR-30d (TAL14)
- miR-16a (TAL14)
- let-7f (TAL14)
- Digest and Run gel
- MAV1212-hEF1a-TAL21
- Flow Cytometry
- TAL14 tuning experiment
- Pick colonies
- miR-125b (TAL14)
- Transform
- Double input low sensor
NOTES
hEF1a-TAL14 and hEF1a-TAL21 both failed digest --> LR again
Can't find double input low sensor --> re golden gate