How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- What about the double input low sensor?
miRNA Sensors (Lab Work)
- Midi prep
- pEXPR: TAL21-mKate
- Run gel
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Golden Gate (if digest works)
- All the TAL21 high sensors
- Gateway (LR)
- pEXPR: hEF1a-TAL21
- Pick colonies
- miR-30d (TAL14)
- miR-125b (TAL14)
- let-7f (TAL14)
- pEXPR: hEF1a-TAL21
- DOX Induction
- L7ae tuning experiment
- Transfect
- TAL14 tuning experiment
NOTES
hEF1a-TAL21 had a red pellet during midi prep spin down --> re-LR & re pick colonies