How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- Include TAL14 and TAL21 high sensors
miRNA Sensors (Theory)
- Analyze sequencing results
- Plan DOX induction experiment for L7ae repression
- Plan to build TAL14/TAL21 high sensors
- Do gating and analysis of Exp 1 Attempt 3
miRNA Sensors (Lab Work)
- Mini prep
- pEXPR: MAV1212-TRE-L7ae
- Digest and run gel
- pEXPR: MAV1212-TRE-L7ae
- Inoculate midi (if digest is okay)
- pEXPR: MAV1212-TRE-L7ae
- Pick colonies
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Low Sensor miR-144-181c
- Golden Gate
- All the target sites & TAL14-mKate with Bbs1
NOTES
we're starting construction of the TAL14/21 high sensors