How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Obtain pEXPR: hEF1a-GAL4VP16
- Fill out the Genewiz Order Form to sequence Low Sensor miR-144
- Prepare transfection well plate maps
- So where are the siRNA though...
- Why didn't the GH golden gate work on try #3?
- Change name of high sensors in tree
miRNA Sensors (Lab Work)
- Pick colonies
- pEXPR: TAL14-mKate
- Midiprep
- pEXPR: MAV1212-Hef11a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- Low Sensor miR-144
- Hef1a-L7ae (LR)
- Miniprep
- MAV1212-mRFP
- L1_TALER14_L2
- pTAL14-mKate-pEST
- LR
- pEXPR: MAV1212-TRE-L7ae
NOTES
so...the siRNA are nowhere to be found
hEF1a-TAL14 (LR) has no colonies
TRE is nowhere to be found