hEF1a:CD79A-pTet:mRFP-Gal4VP16 minipreps:
| Replicate | Concentration |
|---|---|
| h:CDA-pT:RFP-G4 1 | |
| h:CDA-pT:RFP-G4 2 | |
| h:CDA-pT:RFP-G4 3 | |
| h:CDA-pT:RFP-G4 4 | |
| h:CDA-pT:RFP-G4 5 |
hEF1a:CD79A-pTet:mRFP-Gal4VP16 restriction digest verification:
Enzyme used: BamH1
| Expected | Ladder | Gel | Notes |
|---|---|---|---|
 |  |  | Lanes are Ladder, hEF1a:CD79A-pTet:mRFP-Gal4VP16 sample 1, 2, 3, 4, 5. Only samples 1 and 5 appear to have succeeded. ^ that makes sense because only samples 1 and 5 had (very) red bacteria when I spun them down for the miniprep (even though I picked 5 red colonies):
It's interesting that we don't see the lower size bands (i guess that could be because the gel is very jank towards the end) |
