June 9
Plan of Action
- Build hef1+lilrb2
- design cloning strategy
- acquire promoter
- acquire pdest
- build pentr lilrb2
- resuspend gblocks
- acquire gg domd
- run a golden gate
Questions:
- How do we turn a gblock into an entry vector?
- Can we get beta-amyloid in both monomer and oligomer form instead of just monomers that we would have to oligomerize?
- Do we need the cells to be expressing GFP?
June 10
Answers:
- We will work on that later today
- No preference
- Makes for pretty pictures and not hard to do since we're already transfecting a plasmid