How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Find new miRNA target site vector. Email:
- Kyle
- Lila
- Samira
- Obtain plasmid maps
- Hef1a-2xKturn (mutant and w.t.)
- GAL4 (from Brian)
miRNA Sensors (Lab Work)
- Pick colonies & Grow in medium
- EF
- GH
- pENTR: L7ae
- pEXPR: Hef1a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- MAV12112-Hef1a-eBFP2 (control)
- Create pENTR: TAL14
- BP Reaction from TAL14
- Transform TAL14
NOTES
GH and pENTR: L7ae didn't grow properly (their plates had almost no agar)
L7ae was re transformed