How To Please Ron/Brain

  • Keep updating the parts tree (include experiments)

miRNA Sensors (Theory)

  • Update experiments page
    • Obtain plasmid maps
  • Email Jin about TAL14 and miRNA target sites
  • Talk to Lyla/Alexa about transfecting later this week
  • Look for plasmids
    • pEXPR: UAS-Gal4-TAL14-eYFP
    • pEXPR: Gal4VP16
    • pEXPR: Hef1a-TAL14
    • pEXPR: Hef1a-2x Kturn-eYFP

miRNA Sensors (Lab Work)

  • Transformations
    • Low Sensor Golden Gates
    • pEXPR: Hef1a-eBFP2 (186 ng/uL)
    • pEXPR: Hef1a-L7ae (96 ng/uL)
  • Pick red colonies and grow in medium!
  • Find concentrations of eBFP2 and L7ae

NOTES

Nelson doesn't know where it is, but he needs it as well, so he's on the hunt

If we're ready, we can transfect very early next week

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