| hEF1a:CD79A | hEF1a:CD79B | hEF1a:Lyn | hEF1a:GMabH | hEF1a:GMabL | hEF1a:CD79A-TCS-G4 | hEF1a:CD79B-TCS-G4 | TRE:Syk-15-TEVp | hEF1a:rtTA | hEF1a:Gal4VP16 | hEF1a-LacO:mKate | TRE:mKate | hEF1a:LacI | TRE:Gal4VP16 | pENTR hEF1a-LacO | pENTR LacI | TRE:Syk | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| Enzyme | ApaI Cuts in hEF1a, CD79A & backbone | ApaI Cuts in hEF1a, and backbone | ApaI, NcoI Cuts in Lyn, hEF1a and backbone | BglII Cuts in GmabH, hEF1a and backbone | KpnI Cuts in GmabL, hEF1a, and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79B, TCS and backbone | PvuII Cuts in syk, tevp and backbone | ApaI Cuts in hEF1a, rtTA3 and backbone | XhoI Cuts in hEF1a, Gal4VP16 and backbone | NheI | PvuII | ApaI | XhoI | ApaI cuts in hEF1a-LacO | ApaI cuts in LacI | PvuII cuts in syk |
| Virtual gel |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | |  |
| Concentration (ng/uL) | 1110.3 | 1420 | 1314.4 | 983.5 | 1359.0 | 1086.1 | 487.2 | 1161.9 | 706.4 | 441.9 | 741.0 | 483.1 | 420.6 | 1265.3 | 229.8 | 196.7 | 321.8 |
| Buffer | |||||||||||||||||
| Enzyme | |||||||||||||||||
| DNA volume | 0.45032874 | 0.352112676 | 0.3804017 | 0.508388409 | 0.367917586 | 0.460362766 | 1.026272578 | 0.430329632 | 0.70781427 | 1.13147771 | 0.674763833 | 1.03498241 | 1.18877794 | 0.395163202 | 2.175805048 | 2.54194204 | 1.55376 |
| H2O volume | 11.54967126 | 11.64788732 | 11.619598 | 11.49161159 | 11.63208241 | 11.53963723 | 10.97372742 | 11.56967037 | 11.2921857 | 10.86852229 | 11.32523617 | 10.9650176 | 10.8112221 | 11.6048368 | 9.824194952 | 9.45805796 | 10.44624 |
| Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| 1-10 | 11-17 | A-J | K-Q |
 |  |  |  |
hEF1a:CD79A Correct hEF1a: CD79B Correct hEF1a:Lyn Correct hEF1a: GmabH Correct hEF1a:GmabL Correct hEF1a:CD79A-TCS-G4 Correct hEF1a:CD79B-TCS-G4 I can't tell TRE:Syk-15-TEVp Correct hEF1a:rtTA Correct hEF1a:Gal4VP16 ??? | hEF1a-LacO:mKate Correct TRE:mKate Doesn't match either condition hEF1a:LacI Correct TRE:Gal4VP16 Correct pENTR hEF1a-LacO Correct pENTR LacI right band pattern but shifted down a bit -> could be a janky gel. Probably correct because hEF1a:LacI TRE:Syk Correct | All failed golden gates | All failed golden gates |
| hEF1a:CD79A-linker-Gal4VP16 | 3T3 | 3T9 | 3T15 | 6T6 | 6T9 | 6T12 | 9T3 | 9T6 | 9T9 | 9T12 | 9T15 | 12T6 | 12T9 | 12T12 | 15T3 | 15T9 | 15T15 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Letter | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q |
| Concentration (ng/uL) | 18 | 38.3 | 35.3 | 25.1 | 31.0 | 26.8 | 209.9 | 402.9 | 175.6 | 249.1 | 311.6 | 29.2 | 27.2 | 32.7 | 17.6 | 19.3 | 23.5 |
| DNA volume | 12 | 12 | 12 | 12 | 12 | 12 | 2.382087 | 1.241003 | 2.84738 | 2.007226 | 1.604621 | 12 | 12 | 12 | 12 | 12 | 12 |
| H2O volume | 0 | 0 | 0 | 0 | 0 | 0 | 9.617913 | 10.759 | 9.15262 | 9.992774 | 10.39538 | 0 | 0 | 0 | 0 | 0 | 0 |
| Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |