PCR of CD79B+stop (no DMSO)

Gel shows unsuccessful PCR of CD79B+stop from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B QX_stop_R

Annealing temperature:  70C

Run using samples after PCR purification.

PCR of CD79B (5% DMSO)

Gel shows unsuccessful PCR of CD79B+stop from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B QX_stop_R

Annealing temperature:  67C

Run using samples after PCR purification.

PCR of CD79B+stop (with betaine)

PCR of CD79B+stop

Gel shows successful PCR of CD79B+stop from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS012 CD79B Q1_F

Reverse primer:  iGEM_AS013 CD79B QX_stop_R

Annealing temperature:  ?

Run using samples after PCR purification.

Restriction Digest Verification of pENTR

7 July 2014:

See gel here