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Fusion proteins:

 

Fixing frameshifts:

(NNN)(NNN)(NNN)(GGGG)(ATG)(NNN)(NNN)(NNN)

  protein1                    Q site             protein2

 

Add nucleotides:

(NNN)(NNN)(NNN)(TWO nucleotides)(GGGG)(ATG)(NNN)(NNN)(NNN)

  protein1                                                       Q site             protein2

OR

(NNN)(NNN)(NNN)(GGGG)(TWO nucleotides)(ATG)(NNN)(NNN)(NNN)

  protein1                    Q site                                                  protein2

 

 

PirB - goldengate the two pieces into a pENTR before PCRing the linkers on.

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