How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
miRNA Sensors (Lab Work)
- Midi prep
- pEXPR: MAV1212-hEF1a-TAL14
- Transform
- pEXPR: MAV1212-TRE-L7ae (LR from last night)
- Proteinase K
- MAV1212-hEF1a-TAL14
- Restriction Digest and run gel
- MAV1212-hEF1a-TAL14
- LR
- MAV1212-hEF1a-TAL21
- Mini prep
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Send for sequencing
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Pick colonies
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- pEXPR: MAV1212-hEF1a-GAL4VP16
- pEXPR: MAV1212-TRE-GAL4VP16
- Seed plates
- L7ae experiment (Take 3)
NOTES
pEXPR: MAV1212-hEF1a-GAL4VP16 and pEXPR: MAV1212-TRE-GAL4VP16 only had blue colonies, which doesn't make sense because they were both verified...
Brian is in the middle of something on his bench, so we won't midi today