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STEPS:
  1. MATERIALS: Ensure that trypsin is completely thawed, but PBS/versene and complete media are cold (do NOT place in warm water bath).
  2. Aspirate off media.
  3. Rinse wells with 500uL of PBS/versene. Aspirate.
  4. Add 200uL of trypsin/EDTA to each well. Incubate at room temperature for 90 seconds, then neutralize with 800uL of cold complete media.
  5. Triturate (pipette up and down) to disperse clumps. Transfer to cytometry tubes. Place tubes in ice (cells must be kept cold; in stasis).
  6. Run FACS.
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