Miniprepped eYFP:Cofilin colonies
Ran digest gel (PstI, ApaI, Cutsmart)
HL, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, HL
2 might have worked? (Invisible lowest band?)
Sent for sequencing.
Otherwise, waiting for Brian's goldengates (Thank you, Brian :) )
Midiprep tomorrow morning. Need help.