PCR of TCS-Gal4VP16

Gel shows successful PCR of TCS-Gal4VP16.

Forward primer:  iGEM KRB 001 F

Reverse primer:  iGEM KRB 002 R

Annealing Temperature:  63C?

Run using samples after PCR purification.

PCR of CD79B-TCS (no DMSO)

Gel shows unsuccessful PCR of CD79B-TCS.

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B Qlinker_R

Annealing temperature:  70C

Run using samples after PCR purification.

PCR of CD79B-TCS (5% DMSO)

Gel shows unsuccessful PCR of CD79B-TCS.

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B Qlinker_R

Annealing temperature:  67C

Run using samples after PCR purification.