Stored in 1% glycerol in same freezer as P. haloplanktis
200 ul into 20 ml TB
Incubate at 34 degrees at 220 rpm in shaker inside incubator in cabinet in Polz lab
Incubate until OD = 0.4-0.45 (should take approx. 5 hours 40 min)
Wash
- Centrifuge at 4500 rpm for 5 min
- Resuspend pellet in Howard Berg medium (suck out supernatant after centrifuging (900 ul) add 900 ul of HB medium
- Repeat x 2