Still looking for assay for FAEEs
How are we relevant?
Algae making biofuel has issues (contamination, economy). We could beat enzymatic hydrolysis because we consolidate the enzyme synthesis and product production in one.
Biobricks!
Are very old and stuck in the past. We should use the biobrick base vector but modify it to be compatible with new cloning methods.
Biobrick vector contains antibiotic resistance marker and an origin of replication, and a number of restriction enzyme sites. We could replace the resistance marker with a resistance gene and a specific ori. Make a biobrick vector where we swap in MoClo (Modular Cloning( sites. MoClo allows simultaneous rather than just sequential operations/digestions.