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Todo Today:

  • Miniprep LRs
  • Digest LRs
  • Also digest pEXPR_hEF1a(tongue)irBYFP
  • Transform PirB-YFP LRs (maybe do treatment's one too...)
  • PCR LilrB2 for YFP and TCSG4
  • Run: Digests, PCRs
  • Cut out PCR bands if worked.

PCRing at 53 degress, 40 seconds.

Digesting with:

pEXPR_hEF1a: LilrB2 XhoI, CS
pEXPR_TREt: LilrB2 XbaI, CS
pEXPR_hEF1a: PirB_YFP ApaI, CS


Order:

HL, pEXPR_hEF1a:LilrB2 (1-4), pEXPR_hEF1a:LilrB2 C, p(??)_PirB_YFP,  PCR_LilrB2(Y) test, PCR_LilrB2(T) test

HL, pEXPR_TREt:LilrB2 (1-4), pEXPR_TREt:LilrB2 C, PCR_LilrB2(Y), PCR_LilrB2(T)


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