Group D: Testing release of Gal4VP16 by Cofilin-TEVp

Controls:

Well 2: Bleed through from eBFP into mKate

Well 3: Leaky expression of Gal4UAS:mKate without the presence of Gal4VP16

Well 4: Background activation of Gal4UAS:mKate without presence of TEVp-Cofilin

Well 1 HEK293	Well 2 HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Dummy 200ng	Well 3 HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng	Well 4 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate 200ng Dummy 200ng	Well 5	Well 6
Well 7 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 0nM DOX	Well 8 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 1nM DOX	Well 9 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 10nM DOX	Well 10 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 100nM DOX	Well 11 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 1000nM DOX	Well 12 HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin 200ng Gal4UAS:mKate 200ng 2000nM DOX
Well 13	Well 14	Well 15	Well 16	Well 17	Well 18
Well 19	Well 20	Well 21	Well 22	Well 23	Well 24