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  • Miniprep pEXPRs (TREt TC, hEF1a YC)
  • Check pEXPRs (TREt TC, hEF1a YC)
  • Inoculate Midipreps
  • Pick colonies for hEF1a LT, PT, TC

Digest TREtTC with ApaI Cutsmart
Digest hEF1aYC with BsrGI 2.1
Digest LT with BglI 3.1

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