Group A: Membrane Localization
Group B: Receptor Affinity for Beta-amyloid
Group C: Levels of Endogenous Cofilin
Group D: Cofilin Recruitment
- Find data on receptor-cofilin dynamics (esp. how long cofilin stays recruited at the membrane)
- pEXPR hEF1a:cofilin-eYFP
- look at structure of cofilin to determine which (N or C) terminus to put the fluorescent protein
- order cofilin gBlock
- look into different types of cofilin
- look for higher intensity at around membrane
- pEXPR hef1a:eBFP
Group E: (Whole System) Cofilin + TEV Protease Recruitment to Receptor + Cleavage Site + Transcription Factor
- pEXPR hEF1a:Lilrb2-TCS-Gal4VP16
- pEXPR TRE:cofilin-TEV
- pEXPR Gal4UAS:eYFP
- pEXPR hEF1a:rtTA3
- pEXPR hEF1a:eBFP transfection marker
- pEXPR TRE:mKate indicator of effect of dox on transcription
How much TEV Protease we need in the system? - indicated by levels of dox
looking for biggest difference in transfer curve between w/ AB and without AB
two different ladders - dox and AB