Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- Include TAL14 and TAL21 high sensors
miRNA Sensors (Theory)
- Analyze sequencing results
- Plan DOX induction experiment for L7ae repression
- Plan to build TAL14/TAL21 high sensors
- Do gating and analysis of Exp 1 Attempt 3
- Re-analyze all of our data in MATLAB (Gary)
- Learn how to acquire MATLAB from Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
- Mini-prep
- pEXPR: MAV1212-TRE-L7ae
- Digest and run gel
- pEXPR: MAV1212-TRE-L7ae
- Inoculate midi (if digest is okay)
- pEXPR: MAV1212-TRE-L7ae
- Pick colonies
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Low Sensor miR-144-181c
- Golden Gate
- All the target sites & TAL14-mKate with Bbs1
NOTES
- Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3
NOTES:
hEF1a: Gal4VP16 2A rtTA3 culture did not grow so we're starting construction of the TAL14/21 high sensors