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| STEP | TEMP | TIME |
| Initial Denaturation | 98°C | 30 seconds |
| 35 Cycles | 98°C 60°C64°C 72°C | 10 seconds 30 seconds 60 seconds |
| Final Extension | 72°C | 5 minutes |
| Hold | 4°C |
Ask Brian/Jeremy about changing temps/durations
Electrophoresis Gel
Prepared electrophoresis gel according to protocol
Loaded lanes as follows:
Lane 1: Hyperladder Hyperladder 1kb (5uL)
Lane 2: mRFPmRFP cassette
Lane 3: P5-N R
Lane 4: P5-N C
Include lengths of dna
When loading wells with PCR products, used Parafilm to mix 1uL of 6X Orange dye with 5uL of sample and loaded 5uL of this mixture in a lane. Ran gel at 120V for 30 minutes.
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