Immediate To Do
To do's:
- digests and gels for CD79A linker constructs
- kill TRE:Gal4VP16 LR
- transform TRE:Gal4VP16 LR
- run SDM on gel -> gel extraction
- send CD79B-RFP-G4 for sequencing
- Midiprep (from freezer)
- S9TEV
- B15T15
- Golden Gate
- B9T9
- B15T9
- LR
- hEF1a-CuO:Gmab H
- hEF1a-CuO:Gmab L
- hEF1a-CuO:CD79A
- hEF1a-CuO:CD79B
- hEF1a-CuO:Lyn
- Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)
Sequencing account information:BCR 2.0 To Do
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)
Non-Lab To Do's
- Bug Brian about ordering antibody testing supplies
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Other experimental planning (on experimental page)
- Parts list (excel sheet detailing specs/locations of parts)
- How do we do site directed mutagenesis?
- Literature research:
- kozak sequences for heavy and light chains and CD79s
- truncated TEVp
- other antigens to detect using BCR (we were thinking of small molecule detection)
- inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first)
- Cloning planning
- hEF1a:Syk-pTet:mRFP-TEVp
- variable region
- Is gantenerumab proprietary and can we actually publish with it?