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Cloak

miRG1 (BFP transfection marker) w/ red channel cranked

miRG1 + BACE1 (BFP transfection marker) w/ red channel cranked

 

AL -8/7

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miRG 2-4 expression digest

HL 1Kbp | undig. prod. | ScaI | XbaI 2-4 | miRG 2 A-E | miRG 3 A-E | miRG 4 A-E | HL 1Kbp

 

Hef1a:mKate + Hef1a:eBFP

 

Tre:miRG1 + Hef1a:eBFP + 1000nM Dox

JA - 8/11

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 Sent the B samples of miRNAg2–4 for sequencing

AL - 8/11

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 FRET Data

 

AL - 8/12

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JA - 8/13

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  • Well A4 was not transfected with anything because Hef1a:miRNAg1 was not yet midiprepped.
  • A3 also was not transfected (woops)

HL 1Kbp | undig. prod. | Hef1a:miRG1 A-E