...
PCR of CD79B-TCS (with betaine)
Gel shows unsuccessful PCR of CD79B-TCS from pENTR CD79B (pDONR223).
Forward primer: iGEM_AS001 CD79B Q1_F
Reverse primer: iGEM_AS002 CD79B Qlinker_R
Annealing temperature: ?
Run using samples after PCR purification.
PCR of CD79B-TCS
Gel shows successful PCR of CD79B-TCS from pENTR CD79B (pDONR223).
Forward primer: iGEM_AS012 CD79B Q1_F
Reverse primer: iGEM_AS014 CD79B Qlinker_R
Annealing temperature: ?
Run using samples after PCR purification. Extra bands for CD79B-TCS indicated multiple PCR products, used gel extraction protocol to remove the appropriate band.
Restriction Digest Verification of pENTR
7 July 2014:
See gel here
Correct plasmids | Failed plasmids |
|---|---|
CD79B-TCS-G4 (1) CD79B-TCS-G4 (2) CD79B-TCS-G4 (3) CD79B-TCS-G4 (4) |
|