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Digest of miRNA Generator 1 with ApaLI and XbaI

HL1 Kb | Undig. Prod| ApaLI | XbaI | ApaLI and XbaI A-E

Note: single digest with XbaI should result in a single band at 8 Kbp

 

JA - 7/30

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Transfection notes:

  • 1ug Hef1a:BACE1
  • 1ug TRE:BACE2
  • 2ug Hef1a: rtTA
  • 3ug Hef1a:eBFP
  • 3ug Dummy DNA
  • 500uL diluted OptiMEM-lipofectamine

Neuronal research–primary neuron cultures and APPSwe HEK293:

  • ease of culturing
  • accuracy of models
  • where do we get them
  • pros/cons of use

 

AL -8/5

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miRG1 (BFP transfection marker) w/ red channel cranked

miRG1 + BACE1 (BFP transfection marker) w/ red channel cranked

 

AL -8/7

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miRG 2-4 expression digest

HL 1Kbp | undig. prod. | ScaI | XbaI 2-4 | miRG 2 A-E | miRG 3 A-E | miRG 4 A-E | HL 1Kbp

 

Hef1a:mKate + Hef1a:eBFP

 

Tre:miRG1 + Hef1a:eBFP + 1000nM Dox

JA - 8/11

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 Sent the B samples of miRNAg2–4 for sequencing

AL - 8/11

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 FRET Data

 

AL - 8/12

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JA - 8/13

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  • Well A4 was not transfected with anything because Hef1a:miRNAg1 was not yet midiprepped.
  • A3 also was not transfected (woops)

HL 1Kbp | undig. prod. | Hef1a:miRG1 A-E