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Digest of miRNA Generator 1 with ApaLI and XbaI

HL1 Kb | Undig. Prod| ApaLI | XbaI | ApaLI and XbaI A-E

Note: single digest with XbaI should result in a single band at 8 Kbp

Transfection notes:

• 1ug Hef1a:BACE1
• 1ug TRE:BACE2
• 2ug Hef1a: rtTA
• 3ug Hef1a:eBFP
• 3ug Dummy DNA
• 500uL diluted OptiMEM-lipofectamine

Neuronal research–primary neuron cultures and APPSwe HEK293:

• ease of culturing
• accuracy of models
• where do we get them
• pros/cons of use

miRG1 (BFP transfection marker) w/ red channel cranked

miRG1 + BACE1 (BFP transfection marker) w/ red channel cranked

miRG 2-4 expression digest

HL 1Kbp | undig. prod. | ScaI | XbaI 2-4 | miRG 2 A-E | miRG 3 A-E | miRG 4 A-E | HL 1Kbp

Hef1a:mKate + Hef1a:eBFP

Tre:miRG1 + Hef1a:eBFP + 1000nM Dox

 Sent the B samples of miRNAg2–4 for sequencing

 FRET Data

• Well A4 was not transfected with anything because Hef1a:miRNAg1 was not yet midiprepped.
• A3 also was not transfected (woops)

HL 1Kbp | undig. prod. | Hef1a:miRG1 A-E