| tube name | Hyper Ladder 1kb | A/B 1 | A/B 1 | A/B 2 | A/B 3 | A/B 4 | A/B 5 | A4/B 1 | A4/B 1 | A4/B 2 | A4/B 3 | A4/B 4 | A4/B 5 | A/B4 1 | A/B4 1 | A/B4 2 | A/B4 3 | A/B4 4 | A/B4 5 | Hyper Ladder 1kb | |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | |
| DNA concentration (ng/ul) | 154.9 | 154.9 | 441.6 | 321.3 | 242.1 | 332.2 | 432.6 | 432.6 | 537.4 | 484.5 | 488.6 | 478.3 | 229.5 | 229.5 | 153.7 | 379.2 | 490.6 | 270.6 | |||
| Enzyme Name | undigested | NsiI | NsiI | NsiI | NsiI | NsiI | undigested | NsiI | NsiI | NsiI | NsiI | NsiI | undigested | NsiI | NsiI | NsiI | NsiI | NsiI | |||
| Buffer Name | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | |||
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
| DNA volume | #DIV/0! | 3.23 | 3.23 | 1.13 | 1.56 | 2.07 | 1.51 | 1.16 | 1.16 | 0.93 | 1.03 | 1.02 | 1.05 | 2.18 | 2.18 | 3.25 | 1.32 | 1.02 | 1.85 | #DIV/0! | |
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
| enzyme volume | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| strip tube # | 1/1 | 1/2 | 1/3 | 1/4 | 1/5 | 1/6 | 1/7 | 1/8 | 1/9 | 1/10 | 1/11 | 1/12 | 2/1 | 2/2 | 2/3 | 2/4 | 2/5 | 2/6 | 2/7 | 2/8 | |
| water volume | #DIV/0! | 13.8 | 13.8 | 15.9 | 15.4 | 14.9 | 15.5 | 15.8 | 15.8 | 16.1 | 16.0 | 16.0 | 16.0 | 14.8 | 14.8 | 13.7 | 15.7 | 16.0 | 15.2 | #DIV/0! | |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
| loading dye | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | |
| results | all fail-> |
smear | |||||||||||||||||||
| notes | digested | digested |
| smear | These have fresh NsiI | ||||||||||||||||
| Hyper Ladder 1kb | A4/B4 1 | A4/B4 1 | A4/B4 2 | A4/B4 3 | A4/B4 4 | A4/B4 5 | TRE:CD79A | TRE:CD79A | TRE:CD79B | TRE:CD79B | TRE:GmabL | TRE:GmabL | TRE:GmabH 1 | TRE:GmabH 1 | TRE:GmabH 2 | TRE:GmabH 3 | TRE Lyn-mKate | TRE Lyn-mKate | Hyper Ladder 1kb |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
| 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 |
| 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| 451.2 | 451.2 | 88 | 481.9 | 136.9 | 110.7 | 174.4 | 174.4 | 223 | 223 | 184 | 184 | 131 | 131 | 154.1 | 185.4 | 277 | 277 | ||
| undigested | NcoI | NcoI | NcoI | NcoI | NcoI | undigested | StuI | undigested | BamHI | undigested | BamHI | undigested | StuI | StuI | StuI | undigested | bglI | ||
| 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | cutsmart | cutsmart | cutsmart | cutsmart | cutsmart | cutsmart | cutsmart | cutsmart | cutsmart | cutsmart | 3.1 | 3.1 | ||
| 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
| #DIV/0! | 1.11 | 1.11 | 5.68 | 1.04 | 3.65 | 4.52 | 2.87 | 2.87 | 2.24 | 2.24 | 2.72 | 2.72 | 3.82 | 3.82 | 3.24 | 2.70 | 1.81 | 1.81 | #DIV/0! |
| 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 2/9 | 2/10 | 2/11 | 2/12 | 3/1 | 3/2 | 3/3 | 3/4 | 3/5 | 3/6 | 3/7 | 3/8 | 3/9 | 3/10 | 3/11 | 3/12 | 4/1 | 4/2 | 4/3 | 4/4 |
| #DIV/0! | 15.9 | 15.9 | 11.3 | 16.0 | 13.3 | 12.5 | 14.1 | 14.1 | 14.8 | 14.8 | 14.3 | 14.3 | 13.2 | 13.2 | 13.8 | 14.3 | 15.2 | 15.2 | #DIV/0! |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
| 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| good | good | could be good if extra band is undigested | good?, gels really wavy | good | probably good | probably good | probably good but not as good as the two over there <-- | good | |||||||||||
| looks digested | Poor enzyme choice, geneious had a wrong gel image first time |
| Hyper Ladder 1kb | hef1a:Lyn 2 | hef1a:Lyn 2 | hef1a:Lyn 3 | hef1a:Lyn 4 |
| 41 | 42 | 43 | 44 | 45 |
| 500 | 500 | |||
| Hyper Ladder 1kb | hef1a:Lyn 2 | hef1a:Lyn 2 | hef1a:Lyn 3 | hef1a:Lyn 4 |
| 41 | 42 | 43 | 44 | 45 |
| 500 | 500 | 500 | 500 | 500 |
| 20 | 20 | 20 | 20 | 20 |
| 126.9 | 126.9 | 153.9 | 81.4 | |
| undigested | apaI | apaI | apaI | |
| cutsmart | cutsmart | cutsmart | cutsmart | |
| 41 | 42 | 43 | 44 | 45 |
| #DIV/0! | 3.94 | 3.94 | 3.25 | 6.14 |
| 2 | 2 | 2 | 2 | 2 |
| 1 | 1 | 1 | 1 | 1 |
| 4/5 | 4/6 | 4/7 | 4/8 | 4/9 |
| #DIV/0! | 13.1 | 13.1 | 13.8 | 10.9 |
| 41 | 42 | 43 | 44 | 45 |
| 4 | 4 | 4 | 4 | 4 |
| bad digest | bad digest | bad digest | bad digest | |
| A/B | A/B4 | A4/B | A4/B4 | TRE:CD79A | TRE:CD79B | TRE:GmabL | hef1a:Lyn | TRE:GmabH | TRE:Lyn-mkate |
|---|---|---|---|---|---|---|---|---|---|
 |  |  |  |  |  |  |  |  |  |
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