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Expand
titleSetup

HEK293

Oligomerized Beta Amyloid Oligomerized Beta Amyloid 

Oligomerized Beta Amyloid

Well 10 

HEK293

eBFP

Oligomerized Beta Amyloid

Well 16

HEK293

eYFP

Monomerized Beta Amyloid

Well 17

EMPTY

Well 18

EMPTY

Well 1

HEK293

 

Well 2

HEK293

hEF1a: eBFP

Dummy DNA

Well 3

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

0nM dox

Well 4

HEK293

hEF1a: eBFP

GmabH

GmabL

CD79A

CD79B

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

2000nM dox

Well 5 

EMPTY

 

Well 6 EMPTY

 

Well 7

HEK 293

Anti-IgM

Well 8

HEK293

hEF1a: eBFP

Dummy DNA

Anti-IgM


Well 9

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

0nM dox

Anti-IgM

Well 10

HEK293

hEF1a: eBFP

hEF1a: rtTA

TRE: Lyn-mKate

Dummy DNA

2000nM dox

Anti-IgM

Well 11Well 12

Well 7

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

Well 11 

EMPTY

 

Well 12 

EMPTY

 

Well 13

HEK293

Monomerized Beta Amyloid

Well 14

HEK293

eYFP

Dummy DNA

Monomerized Beta Amyloid

Well 15

HEK293

eYFP

Lyn-mKate

Dummy DNA

Monomerized Beta Amyloid

0nM dox

Well 8

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1nM dox

Well 9

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

10nM dox

Well 10

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

100nM dox

Well 11

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1000nM dox

Well 12

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

2000nM dox

Well 13

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

0nM dox

Anti-IgM

Well 14

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1nM dox

Anti-IgM

Well 15

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

10nM dox

Anti-IgM

Well 16

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

100nM dox

Anti-IgM

Well 17

HEK293

hEF1a: eBFP

hEF1a: rtTA

Well 19

HEK293

Anti IgM

Well 20

HEK293

eYFP

Dummy DNA

Anti IgM

Well 21

HEK293

eYFP

Lyn-mKate

Dummy DNA

Anti IgM

Well 22

HEK293

eYFP

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

1000nM dox

Anti-IgM

Well 23

EMPTY

Well 24

18

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Lyn-mKate

2000nM dox

Anti-IgMEMPTY

Expand
titleExpected Results

Lyn should localize in at least some samples. Hopefully Lyn will only localize if the BCR is active and will not localize if it is inactive.

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