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Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1. 

Timeline

TO DO

Parts Diagram

Lab Notebook

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Experiments

Parts Diagram

Lab Notebook

Seeding 24-well Plate......Transfection......FACS

 

Determine which miRNA has greatest repressor potential for BACE1

see more

Demonstrate ability to down-regulate EP2 expression

see more
Experiment
Experiment

Demonstrate effectiveness of miRNA in down-regulating BACE1

see more

transfection plans

Demonstrate inducible upregulation of BACE2 with -YFP

see more

FRET

 

Purchases

 

ItemLink
BACE2http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140
BACE1http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236
Bace2 activity assayhttps://www.anaspec.com/products/product.asp?id=57836
Bace1 activity assayhttp://www.anaspec.com/products/product.asp?id=57888

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