PCR of CD79B+stop (no DMSO)
Gel shows unsuccessful PCR of CD79B+stop from pENTR CD79B (pDONR223).
...
PCR of CD79B+stop (with betaine)
PCR of CD79B+stop
Gel shows successful PCR of CD79B+stop from pENTR CD79B (pDONR223).
Forward primer: iGEM_AS012 CD79B Q1_F
Reverse primer: iGEM_AS013 CD79B QX_stop_R
Annealing temperature: ?
Run using samples after PCR purification.
Restriction Digest Verification of pENTR
7 July 2014:
See gel here
Correct plasmids | Failed plasmids |
|---|---|
CD79B+stop (2) CD79B+stop (3) CD79B+stop (4) | CD79B+stop (1) |