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Comment: Migration of unmigrated content due to installation of a new plugin
Composition Setup
14-Mar-7
 Toggle Cloak
id3/7

 Cloak
id3/7
LR Reaction:
 Label 
 Gene 
 (fmol) 
 Promoter 
 (fmol) 
 Destinatioin 
 (fmol) 
 LR Clonase(uL) 
 Total Volume 
 JX 1 
 mKate 
 5 
 Het1a-LacO 
 5 
 1-2 
 10 
 1 
 4 
 JX 2 
 rtTA 
 5 
 Het1a 
 5 
 1-2 
 10 
 1 
 4 
14-Mar-14
 Toggle Cloak
id3/14

 
Sample Y166
 Cloak
id3/14
Gibson Reaction Mix
 
 
 
 
 
 DNA 
 I-Sce-1 
 Buffer 
 Water 
 Total 
 Mass (μg) 
 1 
 
 
 
 
 Concentration (ng/μL) 
 166 316 
 
 
 
 
 Volume (μL) 
 3.16 
 1 
 2 
 13.84 
 20 
 Order 
 2 
 4 
 3 
 1 
 
Created Gibson reactio mix. Reactants entered in volume and order noted in table.
14-Mar-16
 Toggle Cloak
id3/16

 Cloak
id3/16
Gibson Reaction Mix for All Samples
 
 Mass (μg) 
 Enzyme (μL) 
 Buffer (μL) 
 Total (μL) 
 
 1 
 1 
 2 
 20 
 
 
 
 
 
 
 
 Con (ng/μL) 
 Vol DNA (μL) 
 Vol H2O (μL) 
 
 1.1 
 151 
 6.024096 
 1 
 2 
 10.9759 
 20 
 
 
 
 
 
 
 Order 
 2 
 4 
 3 
 1 
.62 
 10.38 
 CMU-Nde1 
 1.2 
 267 
 3.75 
 13.25 
 
 1.3 
 141 
 7.09 
 9.91 
 
 2.1 
 652 
 1.53 
 15.47 
 TRE-t-Sta1 
 2.2 
 263 
 3.80 
 13.20 
 
 2.3 
 212 
 4.72 
 12.28 
 
 4.1 
 149 
 6.71 
 10.29 
 TRE-t-Sta1 
 4.2 
 415 
 2.41 
 14.59 
 
 4.3 
 583 
 1.72 
 15.28 
 
 6.1 
 353 
 2.83 
 14.17 
 TRE-t-Sta1 
 6.2 
 593 
 1.69 
 15.31 
 
 6.3 
 361 
 2.77 
 14.23 
 
 7.1 
 448 
 2.23 
 14.77 
 Het1a-Xhol 
 7.2 
 237 
 4.22 
 12.78 
 
 7.3 
 186 
 5.38 
 11.62 
 
 8.1 
 246 
 4.07 
 12.93 
 Het1a-Lac-BamH1 
 8.2 
 182 
 5.49 
 11.51 
 
 8.3 
 871 
 1.15 
 15.85 
 
 9.1 
 410 
 2.44 
 14.56 
 TRE-t-Sta1 
 9.2 
 214 
 4.67 
 12.33 
 
 9.3 
 185 
 5.41 
 11.59 
 
 10.1 
 285 
 3.51 
 13.49 
 CMU-Nde1 
 10.2 
 547 
 1.83 
 15.17 
 
 10.3 
 388 
 2.58 
 14.42 
 
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Gel Figure for Previous Samples: