...
Correct plasmids | Failed plasmids |
|---|---|
Gmab Heavy (1) Gmab Heavy (2) Gmab Heavy (3) Gmab Heavy (4) |
|
Sequencing verification:
| Plasmid | Mutations | Where? |
|---|---|---|
| Gmab Heavy (1) | G45 -> V | two variable, |
| Gmab Heavy (2) | two nonsense | no |
| Gmab Heavy (3) | G207 -> W | both constant region |
| Gmab Heavy (4) | K84 -> M | variable region |
| Gmab Heavy (11) | Y82 -> H | variable region |
| Gmab Heavy (12) | no alignment | |
| Gmab Heavy (13) | P290 -> T | constant region |
| Gmab Heavy (14) | none | worked |
| Gmab Heavy (15) | no alignment | |
| Gmab Heavy (16) | one nonsense | no |
| Gmab Heavy (17) | none | worked |
| Gmab Heavy (18) | none | worked |
| Gmab Heavy (19) | one nonsense, two frameshift | no |
| Gmab Heavy (20) | none | worked |
| Gmab Heavy (21) | no alignment | |
| Gmab Heavy (22) | no alignment |