Gel 1
| Construct | hEF1a:CD79A-3T3-Gal4VP16 | hEF1a:CD79A-3T15-Gal4VP16 | hEF1a:CD79A-6T9-Gal4VP16 | hEF1a:CD79A-9T15-Gal4VP16 | hEF1a:CD79A-12T9-Gal4VP16 | hEF1a:CD79A-12T12-Gal4VP16 |
|---|---|---|---|---|---|---|
| Lane | 1 | 2 | 3 | 4 | 5 | 6 |
| Enzyme | NcoI | NcoI | NcoI | NcoI | NcoI | NcoI |
| Virtual Gel |  | |||||
| Conclusion | Correct | Not Correct | Correct | Correct | Correct | Correct |
| Gel 1 |
|---|
 |
Gel 2
| Construct | hEF1a:Gal4VP16 | hEF1a:CD79A-3T15-Gal4VP16 | hEF1a:CD79A-15T9-Gal4VP16 | hEF1a:CD79A-3T9-Gal4VP16 Midi | hEF1a:CD79A-3T9-Gal4VP16 Midi | hEF1a:CD79A-3T9-Gal4VP16 Midi | hEF1a:CD79A-3T9-Gal4VP16 Midi | hEF1a:CD79A-3T9-Gal4VP16 Midi | hEF1a:CD79A-3T9-Gal4VP16 Midi | GG Donr | RFP PCR |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lane | 1,2 | 3,4 | 5,6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 |
| Enzyme | XhoI | NcoI | NcoI | NcoI | NcoI | NcoI | NcoI | NcoI | NcoI | AarI | - |
| Virtual Gel |  | |
...
...
g4 1
g4 2
3.15 1
3.15 2
15.9 1
15.9 2
3.9
6.6
6.12
9.3
9.6
12.6
ggdonr
 | 800bps | ||||||||||
| Conclusion | 1 - Not Correct 2 - Correct | Correct | Correct | Correct | Correct | Correct | Correct | Correct | Correct | Not Correct | Correct |
| Gel 2 |
|---|
 |
Gel 3
| Construct | hEF1a:CD79A-6T9-Gal4VP16 | hEF1a:CD79A-9T12-Gal4VP16 | hEF1a:CD79A-9T15-Gal4VP16 | hEF1a:CD79A-12T9-Gal4VP16 | hEF1a:CD79A-12T12-Gal4VP16 | GG Donr |
|---|---|---|---|---|---|---|
| Lane | 1 - #1 2 - #2 | 3 | 4 | 5 - #1 6 - #2 | 7 - #1 8 - #2 | 9 - NEB T4 ligase buffer 10 - Promega ligase buffer |
| Enzyme | NcoI | NcoI | NcoI | NcoI | NcoI | AarI |
| Virtual Gel | | | ||||
| Conclusion |
| Gel 3 - both pictures are the same gel at different exposures |
|---|
|
