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DNACon (ng/µL)EnzymeBufferVol DNAMass DNAVol EnzymeVol BufferVol WaterVol Total
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MAV1212-Hef1a-Gal4VP16305.5BsaI3.12.4575012.0014.5520
MAV1212-Hef1a-Gal4VP16 control305.5  2.45750  17.5520
MAV1212-TRE-Gal4VP1674.3BsaI3.110.09750126.9120
MAV1212-TRE-Gal4VP16 control74.3  10.09750  9.9120
MAV1212-TRE-L7ae 58.7BsaI3.112.78750124.2220
MAV1212-TRE-L7ae control58.7  12.78750  7.2220
MAV1212-Hef1a-eBFP2-miR181118.6BsaI3.16.327501210.6820
MAV1212-Hef1a-eBFP2-miR181 control118.6  6.32750  13.6820
MAV1212-Hef1a-eBFP2-miR14449.9BsaI3.115.03750121.97020
MAV1212-Hef1a-eBFP2-miR144 control49.9  15.03750  4.9720
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14-7-19

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DNACon (ng/µL)EnzymeBufferVol DNAMass DNAVol EnzymeVol BufferVol WaterVol Total
Ladder         
MAV1212-TRE-Gal4VP1674.3BsaI3.16.735001210.2720
MAV1212-TRE-Gal4VP16 control74.3  6.73500  13.2720
MAV1212-TRE-L7ae 58.7BsaI3.18.52500128.4820
MAV1212-TRE-L7ae control58.7  8.52500  11.4820
MAV1212-Hef1a-eBFP2-miR181118.6BsaI3.14.225001212.7820
MAV1212-Hef1a-eBFP2-miR181 control118.6  4.22500  15.7820
MAV1212-Hef1a-eBFP2-miR144632.6BsaI3.11.58110001215.4220
MAV1212-Hef1a-eBFP2-miR144 control632.6  1.5811000  18.4220
MAV1212-Hef1a-eBFP250.3BsaI3.19.94500127.0620
MAV1212-Hef1a-eBFP2 control50.3  9.94500  10.0620
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14-7-21

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Why why why why why

Found out that we have no low sensors.

 

 

 

14-7-22

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MAV1212-TRE-L7ae

Incomprehensible bands, recommend re-LR.

 

 

14-7-23

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weeeeee!

 

 

 

14-7-24

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MAV1212-Hef1a-TAL14

EcoRI

 

 

14-7-25

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Golden Gate
Combined Low Sensor

 

 

 

14-7-26

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Midi Prep

MAV1212-Hef1a-GAL4VP16
MAV1212-TRE-GAL4VP16
Single Low Sensors

Transformation
MAV1212-Hef1a-eBFP2-miR144-181c

 

 

14-7-28

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