Versions Compared

Old Version 39

changes.mady.by.user Unknown User (gary_9@mit.edu)

Saved on

compared with

New Version Current

changes.mady.by.user Unknown User (gary_9@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
Comment: Migration of unmigrated content due to installation of a new plugin

...

Expand
titleL7ae Repression
Grown in midi culture
NameLocationConcentration
MAV1212-hEF1a-eBFP2Our -20TC RoomMidi preppedGrown in midi culture
pEXPR:hEF1a-L7aeOur -20TC RoomMidi prepped
pEXPR: CAG-2xKturn-eGFP (wt)TC RoomMidi prepped
pEXPR: hEF1aCAG-2x KturnexKturn-eGFP Our -20(mut)TC RoomMidi preppedGrown in midi culture

 

EXPERIMENTS

  1. hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

Expand
titleTAL14 Repression
NameLocationConcentration
pEXPR: hEF1a-eBFP2Our -20TC RoomMidi preppedGrown in midi culture
pEXPR: hEF1a-Gal4Vp16Gal4VP16Our -20Being LR'dVerified, colony being picked for midi prep
pEXPR: hEF1a-TAL14Out Our -20Grown in Being midi cultureprepped
pEXPR: 5xUAS/4xTAL14bsTAL14-eYFPWith Brian?mKateTC RoomMidi preppedUAS needs to be added somehow?

 

EXPERIMENTS:

  1. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
    1. positive control (hopefully linear BFP/YFP)
  3. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14
    1. Should be blue (minimal yellow)

 

...