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TRANSFECTION PLANS

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titleL7ae Repression
Miniprepped
NameLocationConcentration
MAV1212-hEF1a-eBFP2Our -20TC RoomMidi preppedMiniprepped
pEXPR:hEF1a-L7aeOur -20TC RoomMidi prepped
pEXPR: CAG-2xKturn-eGFP (wt)TC RoomMidi prepped
pEXPR: hEF1aCAG-2x Kturn-eYFPOur -20exKturn-eGFP (mut)TC RoomMidi preppedMiniprepped

 

EXPERIMENTS

  1. hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

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titleTAL14 Repression
NameLocationConcentration
pEXPR: hEF1a-eBFP2Our -20TC RoomMidi preppedMiniprepped
pEXPR: hEF1a-Gal4Vp16With JinGal4VP16Our -20Verified, colony being picked for midi prepWill meet with Jin
pEXPR: hEF1a-TAL14With BrianOur -20Being midi preppedpENTR will be LR'd with Hef1a when obtained
pEXPR: 5xUAS/4xTAL14bsTAL14-eYFPmKateWith SamiraTC RoomMidi preppedUAS needs to be added somehow

 

EXPERIMENTS:

  1. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. hEF1a-eBFP2 & hEF1a-Gal4VP16 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
    1. positive control (hopefully linear BFP/YFP)
  3. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14 & 5xUAS/4xTAL14bs-eYFP
    1. Should be blue (minimal yellow)

 

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