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ProductStatusGeneStarting PlasmidStarting Plasmid ResistanceForward PrimerReverse Primer

Tm

hi/lo (anneal)

PCR's that can go together
CD79AcompleteCD79ApENTR CD79A (pDONR223)spectinomyciniGEM KRB 003 FiGEM KRB 004 R62/61 (61)1
CD79A-TCScompleteCD79ApENTR CD79A (pDONR223)spectinomyciniGEM KRB 003 FiGEM KRB 005 R62/61 (61)1
CD79Bneeds gel verificationnew primers orderedCD79BpENTR CD79B (pDONR223)spectinomyciniGEM_AS001 CD79B Q1_FiGEM_AS002 CD79B QX_stop_R72/70 (70)4
CD79B-TCSneeds gel verificationnew primers orderedCD79BpENTR CD79B (pDONR223)spectinomyciniGEM_AS001 CD79B Q1_FiGEM_AS003 CD79B Qlinker_R72/70 (70)4
TCS-Gal4VP16completeGal4VP16pEXPR 5 – hEF1a-FGFR-mKate-TCS(L)-G4ampicilliniGEM KRB 001 FiGEM KRB 002 R62/60 (63)6
Lynwaiting for primersneed to redoLynpENTR Lyn (pDONR223)spectinomyciniGEM CR 006 FiGEM CR 007 R61/60 (60)2
Lyn-mKatewaiting for primerscompleteLynpENTR Lyn (pDONR223)spectinomyciniGEM CR 006 FiGEM KRB 011 R61/60 (60)2
Lyn-(15aa)waiting for primerscompleteLynpENTR Lyn (pDONR223)spectinomyciniGEM CR 006 FiGEM_AS009 Lyn Qlonglink_R61/60 (60)2
mKateDpnI digest completemKatepENTR_L1_mKate_L2kanamyciniGEM KRB 006 FiGEM KRB 007 R58/61 (58)1
SykcompleteSykpENTR Syk (pDONR223)spectinomyciniGEM_AS004 Syk Q1_FiGEM_AS005 Syk QX_stop_R54/54 (54)3
Syk-eYFPpure, need to gel verifycompleteSykpENTR Syk (pDONR223)spectinomyciniGEM_AS004 Syk Q1_FiGEM KRB 010 R54/54 (54)3
Syk-(15aa)waiting for primersneed to redoSykpENTR Syk (pDONR223)spectinomyciniGEM_AS006 Syk Q1_FiGEM_AS007 Syk Qlonglink_R61/59 (59)2
eYFPDpnI digest completeeYFPpENTR_L1_EYFP_L2kanamyciniGEM KRB 008 FiGEM KRB 009 R59/57 (57)5
(15aa)-TEVpwaiting for primersneeds DpnI digestTEVppENTR_L1_Tev2kanamyciniGEM_AS010 TEVp Qlonglink_FiGEM_AS011 TEVp QX_R60/60 (60)2

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ProductStatusDNAamountswater
pENTER CD79A+stopIn Progress

CD79A+STOP

 .91712.283 
pENTR CD79A-TCS-Gal4VP16In Progress

CD79A-TCS

TCS-Gal4VP16

 

.933

2.262

10.005 
pENTER Syk+STOPIn ProgressSyk+STOP 1.02912.171 
pENTR CD79B-TCS-Gal4VP16Waiting on parts

TCS- Gal4VP16

CD79B-TCS.

  
pENTR CD79B+STOPWaiting on partsCD79B+STOP  
pENTR Gmab HeavyWaiting on partsGmab Heavy  
pENTR Gmab LightWaiting on partsGmab Light  
pENTR Syk-eYFPWaiting on parts

...eYFP

Syk...

  
pENTR Syk-TEVWaiting on parts

Syk...

...TEV

  
pENTR Lyn-TEVWaiting on parts

...TEV

Lyn...

  
pENTR Lyn+STOPWaiting on partsLyn+STOP  
pENTR Lyn-mKateWaiting on parts

Lyn...

...mKate