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- Take pENTR colonies from incubator. Make cell stock. Put in fridge and freezer respectively. (10 am). Did not work.
Monday:
- Make empty liquid cell culture to transform DNA into (~16hours)Miniprep GGDonr (is that a good idea?)
- Pick Cells again and incubate (at 6pm)
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Tuesday:
- Miniprep pENTRs
- Make 5 femptomolal femptomolar working solution
- LR (~ 16 hours min, 24 max)
- Miniprep GGDonr
- PCR (possibly)
June 9
Plan of Action
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