| Gene | Promoter | Expression Vector | Why? | Location/Status | ||
|---|---|---|---|---|---|---|
| GmabH | hEF1a | pZDonor 1-GTW-2 | 1st in Gibson | designed | ||
| GmabL | hEF1a | pZDonor 2-GTW-3 | 2nd in Gibson | designed | ||
| CD79A | hEF1a | pZDonor 3-GTW-4 | 3rd in Gibson? | designed | ||
| CD79A-TCS-Gal4VP16 | hEF1a | pZDonor 3-GTW-4 | 3rd in Gibson? | designed | ||
| CD79B | hEF1a | pZDonor 4-GTW-5 | 4th in Gibson? | designed | ||
| CD79B-TCS-Gal4VP16 | hEF1a | pZDonor 4-GTW-5 | 4th in Gibson? | designed | ||
| Lyn | hEF1a | pZDonor 1-GTW-2 | designed | |||
| Lyn-mKate | Tet | pZDonor 1-GTW-2 | designed | |||
| Syk-eYFP | Tet | pZDonor 2-GTW-3 | designed | |||
| Syk-TEVp | Tet | pZDonor 2-GTW-3 | designed | |||
| eBFP | Tet | pZDonor 1-GTW-2 (already in 1-2) | Control for fluorescencefluorescence for pTet promoter | in someone's freezer | ||
| mKate | GAL4/UAS | pZDonor 1-GTW-2 (already in 1-2) | Test activation by transcription factor (Gal4VP16) | in someone's freezer | ||
| rtTA | hEF1a | pZDonor 1-GTW-2 (already in 1-2) | Required for Tet promoters | in someone's freezer |
Do we want hEF1a - Syk?