...
- biotinylated antibodies (that we can use with the AF594-Streptavidin we already have)
- fluorophore-conjugated antibodies (preferably AF594)
- primary antibodies that we can combine with secondary antibodies we already have (see list here)
Gantenerumab/beta-amyloid: Biotinylated beta-amyloid oligomers + Alexa fluor 594-conjugated Streptavidin
VRC01/gp120:
| Name | Company | Species | Clonality | Valid for | Conjugated? | Price |
|---|---|---|---|---|---|---|
| Anti-HIV1 gp120 antibody (Biotin) (ab53937) | abcam | Goat | Polyclonal | WB, ELISA, IHC-Fr, ICC/IF | Biotin | 100uL/$378 |
HyHEL-10/chicken lysozyme:
| Name | Company | Species | Clonality | Valid for | Conjugated? | Price |
|---|---|---|---|---|---|---|
| Anti-Lysozyme antibody (ab391) | abcam | Rabbit | Polyclonal | WB, IP, ELISA, ICC | No, could use with 594 donkey anti-rabbit secondary | 100ug/$373 |
| lysozyme antibody (FITC) orb189142 | biorbyt | Rabbit | Polyclonal | IF/ICC | FITC | 100ug/$315 2-3wk wait |
Purchase recommendations
| Item | Product name/link | Supplier | Cost | Notes |
|---|---|---|---|---|
| gp120 antigen | gp120 (YU2) (HIV-1/Clade B) (aa 34-518), glycosylated recombinant protein | Eenzyme (near DC, works with NIH) | $259, 50ug | Chose YU2 because this strain was used to obtain basically all SPR binding data in VRC01 paper, this product is produced in 293 cells and has 6x His tag |
| chicken lysozyme | http://biomatik.com/products/productDetail/Lyso-yme--LZM---Chicken--Gallus---two-N-terminal-Tags--His-tag-and-T7-tag.aspx | Biomatik | $223, 10ug | |
| anti-his-AlexaFluor594 | https://www.mblintl.com/product/D291-A59 | MBL | $288, 50ug/50uL |