...
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment | Expected Results | Controls | Protocols | Parts | Completed? |
|---|---|---|---|---|---|
| Assay down-regulation of BACE1 by U6/miRNA | |||||
| Assay transcription factor's effectiveness in promoting expression of miRNA/BACE2 | |||||
Purchases
Experiments
Seeding 24-well Plate......Transfection......FACS
| Experiment |
|---|
Demonstrate effectiveness of miRNA in down-regulating BACE1 |
Demonstrate inducible upregulation of BACE2-YFP |
Purchases
| Item | Link | ||||
|---|---|---|---|---|---|
| Item | Link | Purchased? | |||
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140 | BACE1 (mouse) | |||
| BACE1 (human) | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236 |
Options for Directly Assaying BACE2 Expression
| Link | Cost | CommentsBace2 activity assay | https://www.anaspec.com/products/product.asp?id=57836 | $435 | This is a kit to directly measure BACE2 expression |
|---|---|---|---|---|---|
| Bace1 activity assay | http://www. | sigmaaldrichanaspec.com/ | catalog/search?interface=All&term=bace2&N=0+14574647&mode=match+partialmax&focus=product&lang=en®ion=US$315-$380 | This is a kit to directly measure BACE2 expression | |
| http://www.sciencedirect.com.ezproxyberklee.flo.org/science/article/pii/S1550413111003019; http://www.novusbio.com/gene-id/57393#fq=category%3A%22Peptides%20and%20Proteins%22 | $369 for 10ug of TMEM27 | This assay would involve measuring Tmem27 cleavage by Bace2 | |||
| http://www.pnas.org/content/97/17/9712.long | n/a | This assay would involve measuring A-beta cleavage by Bace2 |
Appendix
data sets